Journal: Frontiers in Neuroscience

Article Title: Oxidative stress and inflammation combine to exacerbate cochlear damage and sensorineural hearing loss in C57BL/6 mice

doi: 10.3389/fnins.2025.1563428

Figure Lengend Snippet: Oxidative stress and inflammatory signals are activated in cochlear sections after administration. (A) Representative 4-HNE immunofluorescence staining images in the cochlea. The 10 μm frozen sections of cochlea from the MD + LPS, MD, and LPS groups (three mice per subgroup) show enhanced 4-HNE (red) compared to the control mice. Relative fluorescence intensity of 4-HNE. n = 3 for each condition. (B) Representative IL-1 immunofluorescence staining images in the cochlea. The frozen sections of the cochlea in the MD + LPS group show enhanced IL-1 (red) compared to the other groups. Relative fluorescence intensity of IL-1. n = 3 for each condition. (C) Representative IL-1R1 immunofluorescence staining images in the cochlea. The frozen sections of the cochlea in the MD + LPS and LPS groups show enhanced IL-1R1 (red) compared to the other groups. Relative fluorescence intensity of IL-1R1. n = 3 for each condition. (D) Representative p-NF-kB immunofluorescence staining images in the cochlea. The frozen sections of the cochlea in the MD + LPS and MD groups show enhanced p-NF-kB (red) compared to the other groups. Relative fluorescence intensity of p-NF-kB. n = 3 for each condition. The pictures above were taken from the base turn of the cochlea. Sections were counterstained with DAPI (blue) to highlight the nuclei. Scale bar: 100 μm. Statistical significance was determined by two-way ANOVA. * p < 0.05; ** p < 0.01; *** p < 0.001; ns: not significant. DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Mice in the MD + LPS group received alternating i.p. injections of MD (300 mg/kg; MedChemExpress, USA, Cat# HY-B1897A) on days 1, 3, and 5, and LPS (1 mg/kg, MedChemExpress, Cat# HY-D1056C2) on days 2, 4, and 6.

Techniques: Immunofluorescence, Staining, Control, Fluorescence

Journal: Frontiers in Neuroscience

Article Title: Oxidative stress and inflammation combine to exacerbate cochlear damage and sensorineural hearing loss in C57BL/6 mice

doi: 10.3389/fnins.2025.1563428

Figure Lengend Snippet: Necroptosis and ferroptosis signaling pathways are activated in cochlear sections after administration. (A) Representative RIPK3 immunofluorescence staining images in the cochlea. The 10 μm frozen sections of cochlea from the MD + LPS group (three mice per subgroup) show enhanced RIPK3 (red) compared to the vehicle controls. Relative fluorescence intensity of RIPK3. n = 3 for each condition. (B) Representative P-RIPK3 immunofluorescence staining images in the cochlea. The frozen sections of the cochlea in the MD + LPS group show enhanced P-RIPK3 (red) compared to the other groups. Relative fluorescence intensity of P-RIPK3. n = 3 for each condition. (C) Representative MLKL immunofluorescence staining images in the cochlea. The frozen sections of cochlea in the MD + LPS, MD and LPS groups show enhanced MLKL (red) compared to the other groups. Relative fluorescence intensity of MLKL. n = 3 for each condition. (D) Representative GPX4 immunofluorescence staining images in the cochlea. The frozen sections of cochlea in the MD + LPS, MD, and LPS groups show decreased GPX4 (red) compared to the vehicle controls. Relative fluorescence intensity of GPX4. n = 3 for each condition. The pictures above were taken from the base turn of the cochlea. Sections were counterstained with DAPI (blue) to facilitate the identification of nuclei. Scale bar: 100 μm. Statistical significance was determined by two-way ANOVA. * p < 0.05; ** p < 0.01; *** p < 0.001; ns: not significant. DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Mice in the MD + LPS group received alternating i.p. injections of MD (300 mg/kg; MedChemExpress, USA, Cat# HY-B1897A) on days 1, 3, and 5, and LPS (1 mg/kg, MedChemExpress, Cat# HY-D1056C2) on days 2, 4, and 6.

Techniques: Protein-Protein interactions, Immunofluorescence, Staining, Fluorescence